Advanced Clinical Flow Cytometry Solutions for Oncology Research
Unlocking Cellular Insights with Precision and Expertise
Flow cytometry is becoming a critical tool in clinical trials, especially in immuno-oncology. Cell therapy such as CAR-T and CAR-NK often utilize flow cytometry to monitor the persistence and differentiation of the cells during treatment.
View Our Preclinical Flow Cytometry Solutions
Multi-color flow cytometry assays allow for the identification and quantification of different cell types in a single heterogeneous sample and provide information about the frequency, phenotypes, or drug binding of specific cell populations.
Flow cytometry assays are a valuable endpoint in clinical trials to help assess a drug or cell therapy’s effect. Whole blood, PBMC, or bone marrow aspirate may be used as samples. We have extensive expertise in these areas:
- Immunophenotyping
- Receptor Occupancy Assays (ROA)
- Biomarker Assay Development
- Evaluation of Cell Surface and Intra-Cellular Markers
- Qualification/Validation of Flow Cytometry Assays
- Manufacturing Release Assays for Cell Therapies
- GLP Flow Cytometry
While it is not a requirement for every clinical flow cytometry assay, we are able to perform work in compliance with Good Laboratory Practice (GLP).
For example, standard TBNK panels are gated through CD45+ Lymphocytes to CD3+ cells, and then to CD4 (T Helper) and CD8 (T Effector) populations. B cells are identified as CD3-CD20+ and NK cells as CD3-CD16+. Examples of Non-Human Primate Panel A and Human Panel B TBNK results are shown below.
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Contact our experts to help advance your drug development with TD2's trusted Flow Cytometry Services.
Capabilities and Assays Include:
Tissue Dissociation/Single-Cell Suspension
- Spleen, Tumors, Lymph nodes
Primary Cell Isolation
- Pan T cells isolation kit, mouse
- CD4+ T cell isolation kit, mouse
- isolation of untouched mouse cells from single-cell suspensions from spleen
Immune Population Phenotypingx
- Panel design 20+ colors
- Whole blood, bone marrow, spleen, and tumor
- Viability dye inclusion
- Surface staining
- Intracellular staining, including:
- Cytoplasmic proteins
- Phospo-proteins (specific buffer set)
- Nuclear transcription factors (specific buffer set)
- Cytokines (specific buffer set)
- T-lymphocyte, B-lymphocyte and Natural Killer cells (TBNK)
- T Regulatory Cells
- T Memory, Naive and Effector Cells
- T Cell Activation
- B Memory and Plasma cells
- Humanized mice panels
- Custom panels
- CAP TBNK Panel
Cancer Cells/Tumor Specific Marker Expressionx
- Viability dye inclusion
- Cell lines and single-cell suspension tumors
- Surface staining
Fluorescent Proteins Expressionx
- Viability dye inclusion
- Cell lines and transfected primary cells
- Surface staining
Cell Cycle Analysisx
- Propidium iodide
- Cell lines
- Solid tumors (under development)
Intracellular Protein Analysisx
- Intracellular Cytokines
- Phosphorylated Proteins
- NK Cell Function
- Basophil Activation Test (BAT)
Additional Resources
Receptor Occupancy
Binding of fluorophore labeled “drug” in relation to decreasing dose of unlabeled “drug”
Receptor Occupancy
PBMC Services
Evaluate responses of PBMCs by profiling the function/phenotype of subpopulations
Immune Population Phenotyping
Immune cell population analysis of subcutaneous CT26 murine colon carcinoma tumors
CT26 SC tumors: Gating strategy T cells and NK cells.
Gating involved identifying the total cell population, and then gate out the doublets, gate out the dead cells, move on to our first marker CD45 which gates out any non-immune cells. The bottom right cytogram shows the different NK cell populations. The far left middle cytogram depicts the two T cell population CD4+ and CD8+. Values are % of total CD45+ cells.
Cancer Cells/Tumor Specific Marker Expression
Cancer cells/tumors specific markers expression
- Viability dye inclusion
- Cell lines and single-cell suspension tumors
- Surface staining
Fluorescent Proteins Expression
Fluorescent proteins expression
- Viability dye inclusion
- Cell lines and transfected primary cells
- Surface staining
Cell Cycle Analysis
Cell cycle analysis A549 cells
- Propidium iodide
- Cell lines
Intracellular Protein Analysis
- Intracellular Cytokines
- Phosphorylated Proteins
- T-Regs
- Phosphoroteins
- Cytokines
Cellular Functional Assays
- NK Cell Function
- Basophil Activation Test (BAT)
