--- title: Advanced Clinical Flow Cytometry Analysis Services description: Discover TD2's advanced clinical flow cytometry services for oncology research. Our platform offers comprehensive immunophenotyping, drug mechanism analysis, and high-throughput assays designed to optimize your drug's therapeutic impact. Tailor your treatment strategies with our expert guidance and cutting-edge technology. image: https://td2inc.com/hubfs/Assets/Images/og-home.jpg --- ×   [![td2-logo](https://td2inc.com/hubfs/td2-logo.png "td2-logo")](https://td2inc.com/?hsLang=en) ![Search Site](https://td2inc.com/hubfs/Assets/Icon/search-icon-1.png) [![td2-logo](https://td2inc.com/hubfs/td2-logo.png "td2-logo")](https://td2inc.com/?hsLang=en) ![Search Site](https://td2inc.com/hubfs/Assets/Icon/search-icon-1.png) # Advanced Clinical Flow Cytometry Solutions for Oncology Research ### Unlocking Cellular Insights with Precision and Expertise [Flow cytometry](https://td2inc.com/lp-flow-cytometry-services?hsLang=en) is becoming a critical tool in clinical trials, especially in immuno-oncology. Cell therapy such as CAR-T and CAR-NK often utilize flow cytometry to monitor the persistence and differentiation of the cells during treatment. [View Our Preclinical Flow Cytometry Solutions](https://td2inc.com/preclinical-flow-cytometry?hsLang=en) Multi-color flow cytometry assays allow for the identification and quantification of different cell types in a single heterogeneous sample and provide information about the frequency, phenotypes, or drug binding of specific cell populations. Flow cytometry assays are a valuable endpoint in clinical trials to help assess a drug or cell therapy’s effect. Whole blood, PBMC, or bone marrow aspirate may be used as samples. We have extensive expertise in these areas: - Immunophenotyping - Receptor Occupancy Assays (ROA) - Biomarker Assay Development - Evaluation of Cell Surface and Intra-Cellular Markers - Qualification/Validation of Flow Cytometry Assays - Manufacturing Release Assays for Cell Therapies - GLP Flow Cytometry [Flow Cytometry FAQ](https://td2inc.com/flow-cytometry-faq?hsLang=en) While it is not a requirement for every clinical flow cytometry assay, we are able to perform work in compliance with Good Laboratory Practice (GLP). For example, standard TBNK panels are gated through CD45+ Lymphocytes to CD3+ cells, and then to CD4 (T Helper) and CD8 (T Effector) populations. B cells are identified as CD3-CD20+ and NK cells as CD3-CD16+. Examples of Non-Human Primate Panel A and Human Panel B TBNK results are shown below. ![Advanced Clinical Flow Cytometry Solutions for Oncology Research](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/2022-01-10_15-28-54.png?width=820&name=2022-01-10_15-28-54.png) ### Request more information about our Flow Cytometry Services Contact our experts to help advance your drug development with TD2's trusted Flow Cytometry Services. ### Capabilities and Assays Include: ![Tissue Dissociation/Single-Cell Suspension](https://td2inc.com/hs-fs/hubfs/Assets/Icon/tissue-dissociation.png?width=65&name=tissue-dissociation.png)**Tissue Dissociation/Single-Cell Suspension** - Spleen, Tumors, Lymph nodes ![Receptor Occupancy](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-10.png?width=65&name=icon-10.png)[**Receptor Occupancy**](https://td2inc.com/receptor-occupancy?hsLang=en)x ![PBMC Services](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-testtube.png?width=65&name=icon-testtube.png)[**PBMC Services**](https://td2inc.com/peripheral-blood-mononuclear-cells?hsLang=en)x ![Primary Cell Isolation](https://td2inc.com/hs-fs/hubfs/Assets/Icon/primary-cell-isolation.png?width=65&name=primary-cell-isolation.png)**Primary Cell Isolation** - Pan T cells isolation kit, mouse - CD4+ T cell isolation kit, mouse - isolation of untouched mouse cells from single-cell suspensions from spleen ![Immune Population Phenotyping](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-analysis-biological.png?width=65&name=icon-analysis-biological.png)[**Immune Population Phenotyping**](https://td2inc.com/immunophenotyping?hsLang=en)x - Panel design 20+ colors - Whole blood, bone marrow, spleen, and tumor - Viability dye inclusion - Surface staining - Intracellular staining, including: - Cytoplasmic proteins - Phospo-proteins (specific buffer set) - Nuclear transcription factors (specific buffer set) - Cytokines (specific buffer set) - T-lymphocyte, B-lymphocyte and Natural Killer cells (TBNK) - T Regulatory Cells - T Memory, Naive and Effector Cells - T Cell Activation - B Memory and Plasma cells - Humanized mice panels - Custom panels - CAP TBNK Panel ![Cancer Cells/ Tumor Specific Marker Expression](https://td2inc.com/hs-fs/hubfs/Assets/Icon/immune-population.png?width=65&name=immune-population.png)**Cancer Cells/Tumor Specific Marker Expression**x - Viability dye inclusion - Cell lines and single-cell suspension tumors - Surface staining ![Fluorescent Proteins Expression](https://td2inc.com/hs-fs/hubfs/Assets/Icon/fluorescent-proteins.png?width=65&name=fluorescent-proteins.png)**Fluorescent Proteins Expression**x - Viability dye inclusion - Cell lines and transfected primary cells - Surface staining ![Cell Cycle Analysis](https://td2inc.com/hs-fs/hubfs/Assets/Icon/cell-cycle.png?width=65&name=cell-cycle.png)**Cell Cycle Analysis**x - Propidium iodide - Cell lines - Solid tumors (under development) ![Intracellular Protein Analysis](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-9.png?width=65&name=icon-9.png)**Intracellular Protein Analysis**x - Intracellular Cytokines - Phosphorylated Proteins ![Cellular Functional Assays](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-dropper.png?width=65&name=icon-dropper.png)[**Cellular Functional Assays**](https://td2inc.com/functional-assays?hsLang=en)x - NK Cell Function - Basophil Activation Test (BAT) ## Additional Resources [![Optimizing Specimen Stability – A Comparative Analysis of Shipping Protocols](https://td2inc.com/hubfs/white-paper-optimizing-specimen-stability-thumb.png)](https://td2inc.com/wp-optimizing-specimen-stability-a-comparative-analysis-of-shipping-protocols?hsLang=en) Flow ## [Optimizing Specimen Stability – A Comparative Analysis of Shipping Protocols](https://td2inc.com/wp-optimizing-specimen-stability-a-comparative-analysis-of-shipping-protocols?hsLang=en) [![Leveraging Flow Cytometry to Drive Better Patient Outcomes in Hematologic Malignancy Clinical Trials](https://td2inc.com/hubfs/leveragin-flow-cytometry.png)](https://blog.td2inc.com/leveraging-flow-cytometry-to-drive-better-patient-outcomes-in-hematologic-malignancy-clinical-trials?hsLang=en) Clinical ## [Leveraging Flow Cytometry to Drive Better Patient Outcomes in Hematologic Malignancy Clinical Trials](https://blog.td2inc.com/leveraging-flow-cytometry-to-drive-better-patient-outcomes-in-hematologic-malignancy-clinical-trials?hsLang=en) [![Immunophenotyping Pig Immune Cells by Flow Cytometry: A Powerful Tool for Pharmacology and Toxicology Studies](https://td2inc.com/hubfs/Assets/Images/flow-thumb-9.png)](https://td2inc.com/lp-fcsl-poster-2024-immunophenotyping-pig-immune-cells-by-flow-cytometry-a-powerful-tool-for-pharmacology-and-toxicology-studies/?hsLang=en) Flow ## [Immunophenotyping Pig Immune Cells by Flow Cytometry: A Powerful Tool for Pharmacology and Toxicology Studies](https://td2inc.com/lp-fcsl-poster-2024-immunophenotyping-pig-immune-cells-by-flow-cytometry-a-powerful-tool-for-pharmacology-and-toxicology-studies/?hsLang=en) [![Flow Cytometry Application in Water Microbiology: Testing Bacterial Content in Water](https://td2inc.com/hubfs/Assets/Images/flow-thumb-8.png)](https://td2inc.com/lp-fcsl-poster-2024-flow-cytometry-application-in-water-microbiology/?hsLang=en) Flow ## [Flow Cytometry Application in Water Microbiology: Testing Bacterial Content in Water](https://td2inc.com/lp-fcsl-poster-2024-flow-cytometry-application-in-water-microbiology/?hsLang=en) [![Flow Cytometry Processing and Analysis of Tumor Specimens: Testing Viability and Staining Post Shipping](https://td2inc.com/hubfs/Assets/Images/flow-thumb-7.png)](https://td2inc.com/lp-fcsl-poster-2024-flow-cytometry-processing-and-analysis-of-tumor-specimens/?hsLang=en) Flow ## [Flow Cytometry Processing and Analysis of Tumor Specimens: Testing Viability and Staining Post Shipping](https://td2inc.com/lp-fcsl-poster-2024-flow-cytometry-processing-and-analysis-of-tumor-specimens/?hsLang=en) [![Flow Cytometry Based Assessment of Innate Immune Function Using Phagocytosis and Reactive Oxygen Species Measurement](https://td2inc.com/hubfs/Assets/Images/flow-thumb-6.png)](https://td2inc.com/lp-fcsl-poster-2024-flow-cytometry-based-assessment-of-innate-immune-function-using-phagocytosis-and-reactive-oxygen-species-measurement/?hsLang=en) Flow ## [Flow Cytometry Based Assessment of Innate Immune Function Using Phagocytosis and Reactive Oxygen Species Measurement](https://td2inc.com/lp-fcsl-poster-2024-flow-cytometry-based-assessment-of-innate-immune-function-using-phagocytosis-and-reactive-oxygen-species-measurement/?hsLang=en) [![Measurement of Cytokine mRNA Expression by Flow Cytometry Using the Branched DNA Technology](https://td2inc.com/hubfs/Assets/Images/flow-thumb-5.png)](https://td2inc.com/lp-fcsl-poster-2024-measurement-of-cytokine-mrna-expression/?hsLang=en) Flow ## [Measurement of Cytokine mRNA Expression by Flow Cytometry Using the Branched DNA Technology](https://td2inc.com/lp-fcsl-poster-2024-measurement-of-cytokine-mrna-expression/?hsLang=en) [![10-Color Flow Cytometry Panels for the Evaluation of T Cell Subsets Such as TH Subsets, T Cell Activation, and T Regulatory Cells for Application in Clinical Trials](https://td2inc.com/hubfs/Assets/Images/flow-thumb-4.png)](https://td2inc.com/lp-fcsl-poster-2024-10-color-flow-cytometry-panels-for-the-evaluation-of-t-cell-subsets/?hsLang=en) Flow ## [10-Color Flow Cytometry Panels for the Evaluation of T Cell Subsets Such as TH Subsets, T Cell Activation, and T Regulatory Cells for Application in Clinical Trials](https://td2inc.com/lp-fcsl-poster-2024-10-color-flow-cytometry-panels-for-the-evaluation-of-t-cell-subsets/?hsLang=en) [![The Advantages of Fluorescent Barcoding of Human Cells Using Flow Cytometry for High Throughput Testing With an Application For Human Clinical Trials](https://td2inc.com/hubfs/Assets/Images/flow-thumb-3.png)](https://td2inc.com/lp-fcsl-poster-2024-the-advantages-of-fluorescent-barcoding-of-human-cells-using-flow-cytometry/?hsLang=en) Flow ## [The Advantages of Fluorescent Barcoding of Human Cells Using Flow Cytometry for High Throughput Testing With an Application For Human Clinical Trials](https://td2inc.com/lp-fcsl-poster-2024-the-advantages-of-fluorescent-barcoding-of-human-cells-using-flow-cytometry/?hsLang=en) [![The Activation Profiling of Stat in Human Whole Blood Samples By Flow Cytometry With an Application for Human Clinical Trials](https://td2inc.com/hubfs/Assets/Images/flow-poster-2.png)](https://td2inc.com/lp-fcsl-poster-2024-the-activation-profiling-of-stat-in-human-whole-blood-samples/?hsLang=en) Flow ## [The Activation Profiling of Stat in Human Whole Blood Samples By Flow Cytometry With an Application for Human Clinical Trials](https://td2inc.com/lp-fcsl-poster-2024-the-activation-profiling-of-stat-in-human-whole-blood-samples/?hsLang=en) Load More ## GET STARTED ## Work with a team who believes in your research as much as you do. Are you ready to start your Flow Cytometry studies? Partner with a collaborative oncology CRO that believes in your treatment as much as you do. Take the first step today and contact our experts. [Get Started Now](https://td2inc.com/contact-us/?hsLang=en) × ![Receptor Occupancy](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-10.png?width=65&name=icon-10.png)**Receptor Occupancy** ### Binding of fluorophore labeled “drug” in relation to decreasing dose of unlabeled “drug” ![Receptor Occupancy](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/receptor-occupancy-1.png?width=780&name=receptor-occupancy-1.png) ![Receptor Occupancy](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-10.png?width=65&name=icon-10.png)Receptor Occupancy × ![PBMC Services](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-testtube.png?width=65&name=icon-testtube.png)**PBMC Services** ### Evaluate responses of PBMCs by profiling the function/phenotype of subpopulations ![PBMC Services](https://td2inc.com/hs-fs/hubfs/Assets/Images/pbmc-isolation-1.png?width=549&name=pbmc-isolation-1.png) × ![Immune Population Phenotyping](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-analysis-biological.png?width=65&name=icon-analysis-biological.png)**Immune Population Phenotyping** ### Immune cell population analysis of subcutaneous CT26 murine colon carcinoma tumors CT26 SC tumors: Gating strategy T cells and NK cells. Gating involved identifying the total cell population, and then gate out the doublets, gate out the dead cells, move on to our first marker CD45 which gates out any non-immune cells. The bottom right cytogram shows the different NK cell populations. The far left middle cytogram depicts the two T cell population CD4+ and CD8+. Values are % of total CD45+ cells. ![Immune Population Phenotyping](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/immune-population-phenotyping.png?width=920&name=immune-population-phenotyping.png) × ![Cancer Cells/ Tumor Specific Marker Expression](https://td2inc.com/hs-fs/hubfs/Assets/Icon/immune-population.png?width=65&name=immune-population.png)**Cancer Cells/Tumor Specific Marker Expression** ### Cancer cells/tumors specific markers expression - Viability dye inclusion - Cell lines and single-cell suspension tumors - Surface staining ![Cancer Cells/Tumor Specific Marker Expression](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/tumor-specific-marker-expression.png?width=920&name=tumor-specific-marker-expression.png) × ![Fluorescent Proteins Expression](https://td2inc.com/hs-fs/hubfs/Assets/Icon/fluorescent-proteins.png?width=65&name=fluorescent-proteins.png)**Fluorescent Proteins Expression** ### Fluorescent proteins expression - Viability dye inclusion - Cell lines and transfected primary cells - Surface staining ![Fluorescent Proteins Expression](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/fluorescent-proteins-expression.png?width=920&name=fluorescent-proteins-expression.png) × ![Cell Cycle Analysis](https://td2inc.com/hs-fs/hubfs/Assets/Icon/cell-cycle.png?width=65&name=cell-cycle.png)**Cell Cycle Analysis** ### Cell cycle analysis A549 cells - Propidium iodide - Cell lines ![Cell Cycle Analysis](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/cell-cycle-analysis.jpg?width=920&name=cell-cycle-analysis.jpg) × ![Intracellular Protein Analysis](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-9.png?width=65&name=icon-9.png)**Intracellular Protein Analysis** - Intracellular Cytokines - Phosphorylated Proteins - T-Regs - Phosphoroteins - Cytokines ![Intracellular Protein Analysis](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/intracelluar-1.png?width=475&name=intracelluar-1.png) × ![Cellular Functional Assays](https://td2inc.com/hs-fs/hubfs/Assets/Icon/icon-dropper.png?width=65&name=icon-dropper.png)**Cellular Functional Assays** - NK Cell Function - Basophil Activation Test (BAT) ![Cellular Functional Assays](https://td2inc.com/hs-fs/hubfs/Assets/GraphsFigures/celluar-functional-assays-1.png?width=484&name=celluar-functional-assays-1.png) [![td2-logo-footer](https://td2inc.com/hs-fs/hubfs/td2-logo.png?width=160&name=td2-logo.png)](https://td2inc.com/?hsLang=en) Office 13208 E. Shea Blvd, Suite 100 Scottsdale, AZ 85259 Phone (602) 358-8300 Connect with us - - ![td2-logo-footer](https://td2inc.com/hs-fs/hubfs/Assets/Logo/acclac-logo.png?width=139&name=acclac-logo.png) © 2026 Translational Drug Development. All Rights Reserved. [Terms and Conditions](https://td2inc.com/terms-and-conditions?hsLang=en) | [Privacy Policy](https://td2inc.com/privacy-policy?hsLang=en)