Advanced Preclinical Flow Cytometry Analysis Services

Precision Immunophenotyping & Drug Impact Analysis for Oncology Research

Gain a deeper understanding of your drug’s therapeutic effects through high-throughput flow cytometry analysis across a diverse array of assays and cell types. TD2’s cutting-edge platform offers comprehensive immunophenotyping, enabling you to delve into your drug’s mechanism of action and pharmacodynamics, ultimately strengthening the efficacy of your immunotherapy while effectively minimizing unwanted side effects.

View Our Clinical Flow Cytometry Solutions

Features & Benefits:

  • In-Depth Analysis: Explore immune composition within vital organs such as tumors, spleen, and lymph nodes, allowing you to tailor treatment strategies for maximum impact.
  • Precise Impact Assessment: Evaluate the therapy’s influence on the frequencies and functions of specific cell subsets, optimizing its performance.
  • Expert Guidance: Access our dedicated FACS team’s expertise to customize protocols, panel configurations, gating strategies, and more, ensuring the most relevant results for your study.
  • Efficient High-Throughput: Characterize cells with ease, using minimal sample volumes, thanks to our advanced equipment, including Miltenyi gentleMACS™ Octo Dissociator, MultiMACS Cell24 Separator, Miltenyi MACSQuant Analyzer 16, BD FACSCanto™, BD FACSCanto™ II, 10-color BD FACSCanto, Cytek Aurora, and Millipore Guava PCA

With TD2’s flow cytometry platform, you can confidently navigate the complexities of immune phenotyping and rare cell populations, gaining valuable insights into your drug’s potential and optimizing its therapeutic impact

Capabilities and Assays Include:

Tissue Dissociation/Single-Cell SuspensionTissue Dissociation/Single-Cell Suspension

  • Spleen, Tumors, Lymph nodes

Receptor OccupancyReceptor Occupancyx

PBMC ServicesPBMC Servicesx

Primary Cell IsolationPrimary Cell Isolation

  • Pan T cells isolation kit, mouse
  • CD4+ T cell isolation kit, mouse
    • isolation of untouched mouse cells from single-cell suspensions from spleen

Immune Population PhenotypingImmune Population Phenotypingx

  • Panel design 20+ colors
  • Whole blood, bone marrow, spleen, and tumor
  • Viability dye inclusion
  • Surface staining
  • Intracellular staining, including:
    • Cytoplasmic proteins
    • Phospo-proteins (specific buffer set)
    • Nuclear transcription factors (specific buffer set)
    • Cytokines (specific buffer set)
  • T-lymphocyte, B-lymphocyte and Natural Killer cells (TBNK)
  • T Regulatory Cells
  • T Memory, Naive and Effector Cells
  • T Cell Activation
  • B Memory and Plasma cells
  • Humanized mice panels
  • Custom panels
  • CAP TBNK Panel

Cancer Cells/ Tumor Specific Marker ExpressionCancer Cells/Tumor Specific Marker Expressionx

  • Viability dye inclusion
  • Cell lines and single-cell suspension tumors
  • Surface staining

Fluorescent Proteins ExpressionFluorescent Proteins Expressionx

  • Viability dye inclusion
  • Cell lines and transfected primary cells
  • Surface staining

Cell Cycle AnalysisCell Cycle Analysisx

  • Propidium iodide
  • Cell lines
  • Solid tumors (under development)

Intracellular Protein AnalysisIntracellular Protein Analysisx

  • Intracellular Cytokines
  • Phosphorylated Proteins

Cellular Functional AssaysCellular Functional Assaysx

  • NK Cell Function
  • Basophil Activation Test (BAT)

Request more information about our Flow Cytometry Services

Contact our experts to help advance your drug development with TD2's trusted Flow Cytometry Services.

Markers and Panels Available

Human Immunophenotyping Panels

Integrated Platform for Nonclinical and Clinical Drug Development

  • We can test a variety of species including human, NHP, rodent, dog, equine; and for a variety of specimen types including whole blood, PBMC, bone marrow, spleen, lymph node, cell lines.
  • Any of the markers/panels can be performed as non-GLP testing. A qualification may be
    performed to determine color combinations.


Model Species Immunopheotyping Panels


  • We can test a variety of species including human, NHP, rodent, dog, equine; and for a variety of specimen types including whole blood, PBMC, bone marrow, spleen, lymph node, cell lines.
  • Any of the markers/panels can be performed as non-GLP testing. A qualification may be performed to determine color combinations.

* Additional panels can be validated to meet your GLP needs

FACS Panels – Mouse Lymphoid Lineage Panels


FACS Panels – Mouse Lymphoid Lineage Panels (under development)


FACS Panels – Mouse Myeloid Lineage Panel


FACS Panels – Mouse Myeloid Lineage Panel (under development)


FACS Panels – Human Lymphoid Lineage Panels (under development)


Preclinical Models and Services


Models and Services

TD2’s service offering is consistently expanding and currently includes:

In vivo and In vitro Characterization

Therapeutic Evaluation:

PK, PD and Biomarkers


Everything needed for successful drug development.

Combining early PK with POC efficacy studies, we provide alignment of compound kinetics from in vitro metabolism through PD and PK testing. TD2 offers Proof of Concept (POC) for both small and large molecules of interest and provides support in the following areas:

  • Pharmacokinetic noncompartmental analysis using Phoenix WinNonlin software
  • Tissue distribution
  • Analysis of large molecules
  • Dried Blood Spot (DBS) analysis
  • LC-MS/MS and UV-VIS dose confirmation, in support of dose escalation studies

A Complete DMPK laboratory to support your assay needs

A Complete DMPK laboratory to support your assay needs.

When you partner with TD2, you can be confident that your sample analyses and results are highly reproducible and accurate. We can help tailor your DMPK panel to meet your therapeutic candidate’s requirements. Our DMPK laboratory, located within our AAALAC-accredited, state-of-the-art vivarium, is the ideal environment for cancer drug discovery to properly assesses quantitative pharmacokinetic testing and toxicokinetic analyses.

We provide bioanalytical method development and/or transfer under a non-GLP environment with proper evaluation based on FDA guidance.

DMPK Services +

Our instrumentation includes one of the most highly sensitive LC-MS/MS equipment available, the Waters Xevo TQ-S and Acquity I-class UPLC system.

Other DMPK services we support:

  • Physiochemical properties including chemical stability
  • Stability in hepatic fractions, such as S9, microsomes and hepatocytes
  • CYP inhibition using microsomes or recombinant enzymes (supersomes)
  • High throughput screening for CYP inhibition
  • Plasma stability
  • Phenotyping in CYP or UGT enzymes
  • PAMPA screening for permeability assessment
  • Protein binding including tissue binding, blood-to-plasma ratio, and plasma protein binding
  • Bioanalytical method development and validation


Advanced biomarker assays and analytical readouts.

Trust your research with a team of oncology experts that keep the end goal in mind. TD2 helps advance screening studies by offering various specialty assays from tumor immune cell infiltrate profiling to quantitation of the effect of therapy on the microbiome. In addition, access a wide range of standard applications through the cutting-edge MesoScale Discovery platform including:

  • Cytokines and chemokines
  • Cell signaling pathways
  • Angiogenesis and hypoxia markers
  • Growth and vascular factors
  • Metabolic markers
  • Cell based assays
  • Phosphoprotein analyses

Advanced biomarker assays and analytical readouts


Work with a team who believes in your research as much as you do.

Are you ready to start your Flow Cytometry studies? Partner with a collaborative oncology CRO that believes in your treatment as much as you do. Take the first step today and contact our experts.