Flow Cytometry Services

Gain a deeper understanding of your drug’s therapeutic effects through high-throughput flow cytometry analysis across a diverse array of assays and cell types. TD2’s cutting-edge platform offers comprehensive immunophenotyping, enabling you to delve into your drug’s mechanism of action and pharmacodynamics, ultimately strengthening the efficacy of your immunotherapy while effectively minimizing unwanted side effects.

Features & Benefits:

  • In-Depth Analysis: Explore immune composition within vital organs such as tumors, spleen, and lymph nodes, allowing you to tailor treatment strategies for maximum impact.
  • Precise Impact Assessment: Evaluate the therapy’s influence on the frequencies and functions of specific cell subsets, optimizing its performance.
  • Expert Guidance: Access our dedicated FACS team’s expertise to customize protocols, panel configurations, gating strategies, and more, ensuring the most relevant results for your study.
  • Efficient High-Throughput: Characterize cells with ease, using minimal sample volumes, thanks to our advanced equipment, including Miltenyi gentleMACS™ Octo Dissociator, MultiMACS Cell24 Separator, and Miltenyi MACSQuant Analyzer 16.

With TD2’s flow cytometry platform, you can confidently navigate the complexities of immune phenotyping and rare cell populations, gaining valuable insights into your drug’s potential and optimizing its therapeutic impact

Capabilities and Assays Include:

Tissue Dissociation/Single-Cell Suspension

  • Spleen, Tumors, Lymph nodes

Primary Cell Isolation

  • Pan T cells isolation kit, mouse
  • CD4+ T cell isolation kit, mouse
    • isolation of untouched mouse cells from single-cell suspensions from spleen

Immune Population Phenotyping x

  • Panel design up to 13-colors
  • Whole blood, bone marrow, spleen, and tumor
  • Viability dye inclusion
  • Surface staining
  • Intracellular staining, including:
    • Cytoplasmic proteins
    • Phospo-proteins (specific buffer set)
    • Nuclear transcription factors (specific buffer set)
    • Cytokines (specific buffer set)

Cancer Cells/ Tumor Specific Marker Expression x

  • Viability dye inclusion
  • Cell lines and single-cell suspension tumors
  • Surface staining

Fluorescent Proteins Expression x

  • Viability dye inclusion
  • Cell lines and transfected primary cells
  • Surface staining

Cell Cycle Analysis x

  • Propidium iodide
  • Cell lines
  • Solid tumors (under development)

Request more information about our Flow Cytometry Services

Contact our experts to help advance your drug development with TD2's trusted Flow Cytometry Services.

Panels Available

Models and Services


Models and Services

TD2’s service offering is consistently expanding and currently includes:

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Are you ready to start your preclinical Adoptive Cell Transfer Therapy studies? Partner with a collaborative oncology CRO that believes in your treatment as much as you do. Take the first step today and contact our experts.

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