Advanced Preclinical Flow Cytometry Analysis Services

Precision Immunophenotyping & Drug Impact Analysis for Oncology Research

Gain a deeper understanding of your drug’s therapeutic effects through high-throughput flow cytometry analysis across a diverse array of assays and cell types. TD2’s cutting-edge platform offers comprehensive immunophenotyping, enabling you to delve into your drug’s mechanism of action and pharmacodynamics, ultimately strengthening the efficacy of your immunotherapy while effectively minimizing unwanted side effects.

View Our Clinical Flow Cytometry Solutions

Features & Benefits:

  • In-Depth Analysis: Explore immune composition within vital organs such as tumors, spleen, and lymph nodes, allowing you to tailor treatment strategies for maximum impact.
  • Precise Impact Assessment: Evaluate the therapy’s influence on the frequencies and functions of specific cell subsets, optimizing its performance.
  • Expert Guidance: Access our dedicated FACS team’s expertise to customize protocols, panel configurations, gating strategies, and more, ensuring the most relevant results for your study.
  • Efficient High-Throughput: Characterize cells with ease, using minimal sample volumes, thanks to our advanced equipment, including Miltenyi gentleMACS™ Octo Dissociator, MultiMACS Cell24 Separator, Miltenyi MACSQuant Analyzer 16, BD FACSCanto™, BD FACSCanto™ II, 10-color BD FACSCanto, Cytek Aurora, and Millipore Guava PCA

With TD2’s flow cytometry platform, you can confidently navigate the complexities of immune phenotyping and rare cell populations, gaining valuable insights into your drug’s potential and optimizing its therapeutic impact

Capabilities and Assays Include:

Tissue Dissociation/Single-Cell Suspension

  • Spleen, Tumors, Lymph nodes

Receptor Occupancyx

PBMC Servicesx

Primary Cell Isolation

  • Pan T cells isolation kit, mouse
  • CD4+ T cell isolation kit, mouse
    • isolation of untouched mouse cells from single-cell suspensions from spleen

Immune Population Phenotyping x

  • Panel design 20+ colors
  • Whole blood, bone marrow, spleen, and tumor
  • Viability dye inclusion
  • Surface staining
  • Intracellular staining, including:
    • Cytoplasmic proteins
    • Phospo-proteins (specific buffer set)
    • Nuclear transcription factors (specific buffer set)
    • Cytokines (specific buffer set)
  • T-lymphocyte, B-lymphocyte and Natural Killer cells (TBNK)
  • T Regulatory Cells
  • T Memory, Naive and Effector Cells
  • T Cell Activation
  • B Memory and Plasma cells
  • Humanized mice panels
  • Custom panels
  • CAP TBNK Panel

Cancer Cells/ Tumor Specific Marker Expression x

  • Viability dye inclusion
  • Cell lines and single-cell suspension tumors
  • Surface staining

Fluorescent Proteins Expression x

  • Viability dye inclusion
  • Cell lines and transfected primary cells
  • Surface staining

Cell Cycle Analysis x

  • Propidium iodide
  • Cell lines
  • Solid tumors (under development)

Intracellular Protein Analysis x

  • Intracellular Cytokines
  • Phosphorylated Proteins

Cellular Functional Assays x

  • NK Cell Function
  • Basophil Activation Test (BAT)

Request more information about our Flow Cytometry Services

Contact our experts to help advance your drug development with TD2's trusted Flow Cytometry Services.

Markers and Panels Available

Integrated Platform for Nonclinical and Clinical Drug Development

  • We can test a variety of species including human, NHP, rodent, dog, equine; and for a variety of specimen types including whole blood, PBMC, bone marrow, spleen, lymph node, cell lines.
  • Any of the markers/panels can be performed as non-GLP testing. A qualification may be
    performed to determine color combinations.

  • We can test a variety of species including human, NHP, rodent, dog, equine; and for a variety of specimen types including whole blood, PBMC, bone marrow, spleen, lymph node, cell lines.
  • Any of the markers/panels can be performed as non-GLP testing. A qualification may be performed to determine color combinations.

* Additional panels can be validated to meet your GLP needs

Preclinical Models and Services

Preclinical

Models and Services

TD2’s service offering is consistently expanding and currently includes:

Get Started

Work with a team who believes in your research as much as you do.

Are you ready to start your Flow Cytometry studies? Partner with a collaborative oncology CRO that believes in your treatment as much as you do. Take the first step today and contact our experts.

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