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title: "LP – FCSL Poster 2024: Measurement of Cytokine mRNA Expression by Flow Cytometry Using the Branched DNA Technology - TD2 Precision Oncology"
description: Unlock the potential of Branched DNA Technology in flow cytometry for precise measurement of cytokine mRNA expression at a single-cell level. Delve into the innovative methodology behind this cutting-edge approach, offering consistent and reliable results compared to traditional PCR-based assays. Explore our experiment, showcasing increased IL-2 and IFN-γ mRNA expression in activated T cell subsets, revolutionizing our understanding of posttranscriptional events in T cell function. Uncover the versatility of mRNA flow cytometry, enabling high throughput multi-parametric analysis without the need for cell sorting. Discover the advantages, including the ability to compare mRNA levels and protein production in a single tube, and to evaluate gene expression heterogeneity at a single-cell level. Join us at the forefront of flow cytometry research, where mRNA transcript detection meets high precision cellular analysis, opening new avenues for understanding immune function and disease pathogenesis.
image: https://td2inc.com/hubfs/Assets/Images/fcsl-poster-2024-5.png
---
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# Measurement of Cytokine mRNA Expression by Flow Cytometry Using the Branched DNA Technology
**Authors:** Rubina Pal, Jayne Schaubhut, Lynette Brown and Jennifer J. Stewart
Flow Contract Site Laboratory, LLC
**Abstract:** Branched DNA Technology is a recent advancement in flow cytometry which allows detection of mRNA at a single cell level. This technique utilizes a unique approach of hybridization and amplification of the mRNA and thus produces more consistent results than conventional PCR based assays. This method is compatible with the detection of surface and intracellular antigens using fluorochrome conjugated antibodies and eliminates the need of sorting specific cell population to detect mRNA.
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