The Activation Profiling of Stat in Human Whole Blood Samples By Flow Cytometry With an Application for Human Clinical Trials

Authors: Sonal Suhane, Lynette Brown and Jennifer J. Stewart
Flow Contract Site Laboratory, LLC

Abstract: The JAK-STAT signaling pathway is responsible for transmitting information from the cellular cytoplasm to the nucleus, modulating DNA transcription and expression of genes involved in immunity, proliferation and oncogenesis. In the development and progression of certain types of cancers, involvement of a combination of different STAT proteins (STAT1, STAT3 and STAT5) are usually involved. Flow cytometry, a high complexity testing, has become more and more usefully in studying certain pathways due to the development of antibodies and kits that measure the reactions.