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Immune Population Phenotyping

Immune cell population analysis of subcutaneous CT26 murine colon carcinoma tumors

CT26 SC tumors: Gating strategy T cells and NK cells.

Gating involved identifying the total cell population, and then gate out the doublets, gate out the dead cells, move on to our first marker CD45 which gates out any non-immune cells. The bottom right cytogram shows the different NK cell populations. The far left middle cytogram depicts the two T cell population CD4+ and CD8+. Values are % of total CD45+ cells.

Protein Binding Analyses

Protein binding assays assesses the degree to which drugs attaches to proteins within the plasma/tissue. A drug’s efficacy may be affected by the degree to which it binds. TD2 has validated this assay using the Rapid Equilibrium Device and analysis done by LC-MS.

• Plasma
• Microsomal
• Tumor/Tissue

Figure 3. The process of protein binding assessment with rapid equilibrium dialysis.

Figure 4. Protein binding analysis by equilibrium dialysis of plasma
across mutiple different speacies (mouse, rat, dog, monkely, human)
following exposure to multiple compounds.